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Methods and protein design to increase the efficiency and contrast of photo convertible fluorescent proteins

We disclose two fluorescent protein technologies, which we refer to as “Janus” and “Ignis,” respectively. Bothare green-to-red photoconvertible fluorescent proteins (PC-FPs) of the EosFP family, generated through sitedirectedmutagenesis of the mEos4b protein coding sequence at amino acid positions 41 and 70 (Janus) andpositions...

Published: 5/22/2024 | Inventor(s): Brian Stoveken, James Lechleiter

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Category(s): Diagnostics > Assay, Life Sciences Research Tools > Proteomics, Life Sciences Research Tools > Reagents